Antisense Gene Silencing and Bacteriophages as Novel Disinfection Processes for Engineered Systems
نویسندگان
چکیده
Antisense Gene Silencing and Bacteriophages as Novel Disinfection Processes for Engineered Systems
منابع مشابه
The Efficiency of CD40 Down Regulation by siRNA and Antisense ODN: Comparison of Lipofectamine and FuGENE6
Background: Dendritic cells (DCs) are ideal accessory cells in the field of gene therapy. Delivery of DNA and siRNA into mammalian cells is a useful technique in treating various diseases caused by single gene defects. Selective gene silencing by small interfering RNAs (siRNAs) and antisense oligodeoxynucleotides (ODN)s is an efficient method for the manipulation of cellular functions. An effic...
متن کاملBacteriophage Disinfection of Engineered Waste Streams BACTERIOPHAGE DISINFECTION OF ENGINEERED WASTE STREAMS
The threat of bacterial contaminants in water distribution systems and in the environment is of unremitting concern for environmental engineers. This problem is compounded by the overuse of antibiotics and the resulting increased prevalence of antibiotic resistant pathogens in engineered waste streams and hospital wastewater effluents. Through defects in sewer systems these pathogens are furthe...
متن کاملEffect of Heavy Metals on Silencing of Engineered Long Interspersed Element-1 Retrotransposon in Nondividing Neuroblastoma Cell Line
Background: L1 retrotransposons are the most active mobile DNA elements in human genome. Unregulated L1 retrotransposition may have deleterious effect by disrupting vital genes and inducing genomic instabilities. Therefore, human cells control L1 elements by silencing their activities through epigenetic mechanisms. It has been shown that cell division and heavy metals stimulate the frequency of...
متن کاملExpression of RNA-Interference/Antisense Transgenes by the Cognate Promoters of Target Genes Is a Better Gene-Silencing Strategy to Study Gene Functions in Rice
Antisense and RNA interference (RNAi)-mediated gene silencing systems are powerful reverse genetic methods for studying gene function. Most RNAi and antisense experiments used constitutive promoters to drive the expression of RNAi/antisense transgenes; however, several reports showed that constitutive promoters were not expressed in all cell types in cereal plants, suggesting that the constitut...
متن کاملEngineered and construction of pDS132::∆virG as suicide vector for targeted gene deletion of virG from Shigella flexneri 2a in order to generation a live attenuated Shigella vaccine
Background & Objective: Shigella are Gram negative bacteria capable of inducing their entry into non-phagocytic cells via secretion of various effector proteins called invasion plasmid antigens (Ipas). The most important of them is VirG protein. Live attenuated Shigella vaccines have indicated promise in inducing protective immune responses in human clinical trials. In current situation, const...
متن کامل